01339nas a2200217 4500008004100000245011200041210006900153260001500222300001000237490000700247520068800254653001700942653001400959653001200973653001200985653001900997100002401016700001801040700002001058856004301078 2017 eng d00aTwo new distribution records of Aedes (Rusticoidus) refiki Medschid, 1928 (Diptera: Culicidae) from Germany0 aTwo new distribution records of Aedes Rusticoidus refiki Medschi c12/05/2017 a18-240 v353 aAlthough relatively rare, the culicid species Aedes refiki is considered widely distributed throughout Europe. It has also been described to occur over large parts of Germany, but reports are scarce and date back several decades. The last time Ae. refiki was documented for Germany was in 1980 when the species was found in the central northern part of the country. During larval sampling activities, Ae. refiki was rediscovered at two locations in the German federal state of Thuringia in spring 2016. The collection sites, method of species identification and species characteristics are described and discussed. Journal of the European Mosquito Control Association 35: 18-24, 201710aAedes refiki10aCulicidae10aecology10aGermany10aidentification1 aKuhlisch, Cornelius1 aKampen, Helge1 aWalther, Doreen uhttps://e-m-b.myspecies.info/node/115602071nas a2200241 4500008004100000245007500041210006900116260001500185300000800200490000700208520138700215653000801602653001401610653001801624653003101642653001701673653001201690100001801702700002501720700001801745700002301763856004301786 2017 eng d00aSpecies identification of Swedish mosquitoes through DNA metabarcoding0 aSpecies identification of Swedish mosquitoes through DNA metabar c16/02/2016 a1-90 v353 aAbstract: DNA-barcoding utilises a fragment of the mitochondrial cytochrome oxidase subunit 1 (COI) gene to identify most animal species. Using next generation sequencing (NGS), this method can be further developed into metabarcoding processes that allow the simultaneous identification of several species from a mixed sample. We created a database of COI sequences of 27 mosquito species collected in Sweden, and combined our data with 27 additional sequences from GenBank to cover the taxa recently documented in Sweden and to include possible invasive taxa. Comparisons show that COI metabarcoding reliably identifies 41 of 54 species and the remainder to species group. Using three independent primer pairs along the COI gene, we further developed this barcoding approach to simultaneously identify Swedish mosquitoes in communities using NGS and quantify relative abundance of each mosquito species in the sample, using bioinformatics methods. We tested the accuracy of the metabarcoding method using communities assembled from morphologically identified mosquitoes, revealing 80% positive identification rate and the estimates of population structure which reflects the input sample. We conclude that metabarcoding is useful as a high throughput identification technique and for the quantification of species. Journal of the European Mosquito Control Association 35: 1-9, 201710aCOI10aCulicidae10ametabarcoding10anext generation sequencing10asurveillance10avectors1 aLilja, Tobias1 aNylander, Johan, A.A1 aTroell, Karin1 aLindström, Anders uhttps://e-m-b.myspecies.info/node/115202003nas a2200241 4500008004100000245013000041210006900171260001500240300001000255490000700265520128200272653001901554653001801573653001401591653001501605653001501620653001001635653002201645100001601667700001301683700002201696856004301718 2015 eng d00aBlood-feeding Diptera (Culicidae and Ceratopogonidae) in an urban park of the city of Vitoria-Gasteiz (Basque Country, Spain)0 aBloodfeeding Diptera Culicidae and Ceratopogonidae in an urban p c06/07/2015 a10-140 v333 aHaematophagous and synanthropic Diptera are responsible for the transmission of several pathogenic agents to humans, domestic and wild animals, causing diverse vector-borne diseases of paramount importance. The presence of these blood-feeding insects is well reported in many areas of the world; however, the distribution in anthropogenic zones has not been studied so far across Spain. In order to gather information on this entomofauna in urban environments, adult stages were collected using an automatic entomological aspirator during the summer season in street lamps of a public park located in the city of Vitoria-Gasteiz (Basque Country, Spain). A total of 333 culicids from four species were collected in a two-year sampling period: Culex pipiens (91.3%), Culiseta longiareolata (8.1%), Culiseta litorea (0.3%) and Culex hortensis hortensis (0.3%). Moreover, 225 Culicoides specimens belonging to six different species were trapped: Culicoides punctatus (61.3 %), Culicoides obsoletus s.s. (16.8%), Culicoides duddingstoni (13.3%), Culicoides kibunensis (6.2%), Culicoides dewulfi (1.7%) and Culicoides clastrieri (0.4%). The first data of Culicoides species distribution in urban areas and the first record of C. litorea are recorded for the Basque Country region. 10aBasque Country10abiting midges10aCulicidae10aCulicoides10amosquitoes10aSpain10aurban environment1 aGonzalez, M1 aLopez, S1 aAlarcon-Elbal, PM uhttps://e-m-b.myspecies.info/node/114601382nas a2200217 4500008004100000245010300041210006900144260001500213300001000228490000700238520074700245653001400992653001001006653001401016653001301030653001901043653001601062653002501078100001801103856004301121 2014 eng d00aInvertebrates found in underground shelters of western Bohemia. I. Mosquitoes (Diptera: Culicidae)0 aInvertebrates found in underground shelters of western Bohemia I c19/12/2014 a27-320 v323 a

Mosquitoes found hibernating in underground shelters were studied in western Bohemia, Czech Republic. Species collected included Culiseta alaskaensis, Cs. annulata, Cs. glaphyroptera, Culex spp., and Anopheles maculipennis s.l.. Culex spp. were the most common, followed by Cs. glaphyroptera and Cs. annulata with few records of Cs. alaskaensis and An. maculipennis s.l.. Culex spp. were found in a variety of shelters, An. maculipennis s.l. preferred cellars, and Cs. alaskaensis where they were found, exhibited little preference. Culiseta annulata and Cs. glaphyroptera were found in similar numbers of cellars, caves, and bunkers, but Cs. glaphyroptera were found more commonly in mines than Cs. annulata.


 

10aAnopheles10aCulex10aCulicidae10aCuliseta10aCzech Republic10ahibernation10aunderground shelters1 aDvorak, Libor uhttps://e-m-b.myspecies.info/node/114202713nas a2200349 4500008004100000245006200041210005800103260001200161300001000173490000700183520173300190653001901923653002201942653001401964653002601978653002602004653001102030100002402041700002602065700002202091700001902113700002302132700002402155700001802179700001802197700001502215700002002230700001702250700001802267700003502285856004302320 2013 eng d00aThe geographic distribution of mosquito species in Sweden0 ageographic distribution of mosquito species in Sweden c11/2013 a21-350 v313 a

Surveillance of the actual distribution of mosquito species in Northern Europe is fundamental for evaluating risk for emerging pathogens, and for research on potential vectors. The Swedish mosquito fauna composition and geographic distribution, originally described by Professor Christine Dahl in the 1970´s, included 43 species. We have compiled the information published from 1978 to 2012, and our own surveillance data from 2001 to 2013, and compared this with the species list and geographic distribution provided in “Taxonomy and geographic distribution of Swedish Culicidae” by Dahl (1977). New species detected during these 36 years were Culiseta (Culicella) ochroptera (Peus, 1935) published 1984, Aedes (Aedes) rossicus Dolbeskin, Goritzkaja & Mitrofanova, 1930 published 1986, Anopheles (Anopheles) beklemishevi published 1986, Aedes (Ochlerotatus) euedes (Howard, Dyar & Knab, 1912) published 2001, Aedes (Ochlerotatus) nigrinus (Eckstein, 1918) first recorded in 2012, and Anopheles (Anopheles) algeriensis Theobald, 1903, first recorded in 2013. We provide maps with the distribution by province for each species, including historic information up until 1977, and new records from 1978 to 2013, showing the similarities and differences between the old and the new records. Important findings in recent years include the wide distribution of the Sindbis virus enzootic vector Culex (Culex) torrentium Martinii, 1925, and the more limited distribution of the potential West Nile virus vector Culex (Culex) pipiens Linnaeus, 1758. The updated list of mosquito species in Sweden now includes 49 species.

10aCDC Light Trap10aCounter-flow trap10aCulicidae10amosquito distribution10amosquito surveillance10aSweden1 aLundström, Jan, O.1 aSchäfer, Martina, L.1 aHesson, Jenny, C.1 aBlomgren, Eric1 aLindström, Anders1 aWahlqvist, Pernilla1 aHalling, Arne1 aHagelin, Anna1 aAhlm, Clas1 aEvander, Magnus1 aBroman, Tina1 aForsman, Mats1 aVinnersten, Thomas, Z. Persson uhttps://e-m-b.myspecies.info/node/113401785nas a2200169 4500008004100000245010100041210006900142260001200211300001000223520124600233653002601479653001401505653001201519100002101531700002001552856004301572 2013 eng d00aRediscovery of Anopheles algeriensis Theob. (Diptera: Culicidae) in Germany after half a century0 aRediscovery of Anopheles algeriensis Theob Diptera Culicidae in  c01/2013 a14-163 a

Anopheles algeriensis, a mosquito species primarily found in the Mediterranean region and susceptible to Plasmodium spp., is one of seven Anopheles species previously reported from Germany. However, the last record dates back to 1958. Whether it was really absent since then or whether this is just a sign of neglected mosquito studies over several decades is unclear. The present study is part of a nationwide mosquito mapping project launched in 2011. We describe larval dip collections of An. algeriensis in 2013 from the inland salt meadow “Brenner Moor”, located in Schleswig-Holstein, northern Germany, which represents the most northerly of all German An. algeriensis collection sites known so far. Despite several mosquito mapping projects carried out in Germany over recent years, An. algeriensis has not been detected within the last 55 years. Therefore this species appears to be extremely rare or at least rurally localised. Hence, it remains a very low risk vector in terms of autochthonous malaria transmission in Germany. Journal of the European Mosquito Control Association 31: 14-16, 2013

10aAnopheles algeriensis10aCulicidae10aGermany1 aKrüger, Andreas1 aTannich, Egbert uhttps://e-m-b.myspecies.info/node/113201814nas a2200205 4500008004100000245013100041210006900172260001200241300000800253520114500261653003001406653001301436653001401449653002301463653002201486100002101508700001901529700001701548856004301565 2013 eng d00aA recent survey of the mosquito (Diptera: Culicidae) fauna and seasonal human biting activity in the city of Chisinau, Moldova0 arecent survey of the mosquito Diptera Culicidae fauna and season c01/2013 a1-73 a

This is the first report on species composition and seasonal human biting activity of mosquitoes in the city of Chisinau, Moldova. In total 3,255 adult mosquitoes were collected in eight recreational areas. Sampling occurred by means of two methods: human landing collection and net-catches from vegetation. Altogether, 22 mosquito species in nine genera were found, representing 55% of the species diversity in Moldova. Seventeen anthropophilic species with different seasonal patterns of biting activity were found in human landing collections. The most frequently captured species was Aedimorphus vexans (N = 1,514; 46.5%) followed by Ochlerotatus annulipes (N = 403; 12.4%), Dahliana geniculata (N = 330; 10.1%), Culex modestus (N = 312; 9.6%), Coquillettidia richiardii (N = 144; 4.4%) and Oc. riparius (N = 117; 3.6%). Eight of the species are the known vectors of West Nile virus in Europe; four of them are the natural vectors of Dirofilaria. Journal of the European Mosquito Control Association 31: 1-7, 2013

10aanthropophilic mosquitoes10aChisinau10aCulicidae10arecreational areas10aseasonal activity1 aSulesco, Tatiana1 aToderas, Lidia1 aToderas, Ion uhttps://e-m-b.myspecies.info/node/1129